Together with optimized qiagen kits for realtime pcr, the rotorgene q enables streamlined analysis for a wide range of applications. Rotor gene q qiagen real time pcr 5plex hrm system. You can perform manual reaction setup, or take advantage of qiagens automated solutions for. This method uses a specially tuned, high intensity optical channel with a thermal resolution of 0. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user. Stateofthe art analyses supported by userfriendly software. Qiagen launches unique calr mutation assay for mpn diagnosis. Type in the serial number six digits, which is located at the back of the rotorgene. Qiagen rotorgene q cycler for realtime pcr and highresolution melting hrm applications by qiagen. Combination with the rotorgene q and qiagen realtime and 2. The rotorgene q uses a sophisticated heating and cooling design to achieve optimal reaction conditions. Rotorgene q mdx 5plex hrm user manual ca 052018 contents3 6. Rotorgene q pure detection 032011 the easytouse software provides. Alternatively, a computer in the assay setup room may be used for run setup.
The rotorgene offers the most userfriendly realtime analysis software system currently available. Please continue to use the older 36position and 72 position rotors with rotorgene 3000 instruments. The 36well rotor and 72well rotor for the rotorgene q are not to be used on rotorgene 3000 instruments due to optical alignment incompatibilities. In addition to hrm channel, this pcr system has 5 color channels, green, yellow, orange, red and crimson. Rotorgene q a rapid, automatable realtime pcr instrument for. Qiagens realtime pcr cycler, the rotorgene q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Rotor gene q handbook readdownload the rotorgene multiplex rtpcr kit is designed for use with the rotorgene q and other rotorgene cyclers, providing ultrafast, highly reliable quantification.
Qiagen rotor gene q 5plex hrm real time pcr available at the lab world group. The rotorgene q, if used in combination with qiagen kits indicated for use with the rotorgene q instrument, is intended for the applications described in the respective qiagen kit handbooks. The newly released rotorgene 3000 uses a world first centrifugal design to provide the researcher with unparalled sensitivity and precision at a fraction of the costs associated with other high end systems. For all you need to run reactions using rotordiscs, choose the rotordisc 100 starter kit or the rotordisc 72 starter kit. Laboratory equipment qiagen rotorgene q mdx installation manual 24 pages laboratory equipment qiagen bluv user manual.
This user guide describes the features of the software. Qiagens realtime pcr cycler, the rotorgene q, combines multiple. The countable reads are those that are used by the variant detection tool when calling the variant. Rotor gene q software manual downloadqiagen rotor gene q user. Cfx96 biorad smartcycler with life science software 2. Qiagen kit handbooks and user manuals are available at or can be. Fixed optical path length led excites from the side of the chamber. Guide to running kasp genotyping reactions on the qiagen rotor.
Fda summary of safety and effectiveness data page 1 summary of safety and effectiveness data ssed i. User guide contractual use of its customer in connection with the use of the products option 3. Print bookmark share for rotor gene q instruments using rotor gene q software version 2. Welcome to the rotorgene 6000 series software version 1. Rotorgene screenclust hrm software is an extension to the rotorgene operating software. Rotorgene q mdx user manual us 022018 contents3 1 operating procedures rotorgene assaymanager software 61 2 access protection 21 2. Limited license agreement for artus cmv rgq mdx kit use of this product signifies the agreement of any purchaser. Check the whatsnew section of this manual for an update on changes and novel features in this version of the software. Rotorgene software manual franks hospital workshop.
Guide to running kasp genotyping reactions on the qiagen. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe. Qiagen rotor gene q 5plex hrm real time pcr youtube. Rotordiscs can be quickly and easily sealed with plastic film using a rotordisc heat sealer. Refer to the rotorgene q mdx instrument user manual for additional warnings, precautions, and procedures. Which reads are countable depends on the user settings when the variant calling is performed if e. Rotorgene q is a program designed for the qiagen rotorgene q device that enables streamlined analysis for a wide range of applications. Should there be an exceptional situation in which we process personal data for the performance of a task carried out in the public interest or in the exercise of official authority vested in us or for reasons necessary for the legitimate interests of our or third parties, you have a right to object to such processing of your personal data, including profiling based on these reasons, and we. Rotorgene assaymanager can also be used for experiment setup, result approval, or reporting on computers not connected to a rotorgene q cycler. Kasp chemistry for allelic discrimination performs well on a qiagen rotorgene q instrument and this stepby step protocol will enable users to successfully. A rt pcr rotor gene was used for blue tongue diagnosis and the curve was absent either the internal control was absent, any suggestions. Qiagen kit handbooks and user manuals are available at. European launch of the new ce marked ipsogen calr rgq pcr kit to improve mpn diagnosis for leukemia patients in line with latest who recommendations and clinical guidelines.
The rotorgene q, if used in combination with qiagen kits indicated for use with the rotor. Qiagen is the leading provider of innovative sample and assay technologies. The rotorgene q has multiple design features providing you with the outstanding performance and reliable results that your research demands. These products are not intended for the diagnosis, prevention or treatment of a disease. Page option determines how the reaction data are to be grouped for analysis purposes after import into the rotorgene q software. This help manual will instruct the user through run setup and analysis. Qiagen rotor gene q 5plex real time pcr with hrm on sale. Do not use components of the kit that have passed their expiration date, or. It combines multiple optimized features to provide powerful performance and reliable results that your research demands. The unique rotary format ensures optimal thermal and optical uniformity between samples which is critical for precise and reliable analysis. The following supplemental instructions will aid in programming an assay template for rotorgene q software version 2.
Please refer to the rotorgene q user manual for complete information about the proper care, maintenance, and use of the rotorgene q cycler. Qiascreen hpv pcr test handbook instructions for use. Lowabundance transcripts are reliably quantified, even when. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. Alteration of incubation times and temperatures may result in erroneous or discordant data. Outstanding thermal and optical performance due to rotary format an unmatched optical range spanning uv to infrared wavelengths stateofthe art analyses supported by user friendly software low maintenance and maximum convenience due to robust design high performance in multiple applications with. This document is intended as a guide to running kasp genotyping reactions on the qiagen rotorgene q instrument. When used with optimized qiagen kits for realtime pcr, the rotorgene q can be used for a wide range of applications. Qiagen rotor gene q software download titleqiagen rotor gene q software download easyartus rg pcr kits cewith easyartus rg pcr kits, qiagen now provides fully integrated automated sample purification and detection of bk virus, cmv, ebv, hsv, and vzv as ce ivd marked solutions, as required, for example, for routine transplantation diagnostics. Together with optimised qiagen kits for realtime pcr, the rotorgene q enables streamlined analysis for.
Qiagens realtime pcr cycler, the rotorgene q, combines multiple optimized design features to provide the outstanding performance and reliabl. In this case, the software can be installed on the user s office computer. Rotorgene q pure detection 062010 comprehensive software and easy maintenance the easytouse software provides. If the rotorgene q instrument is used with kits other than qiagen kits, it is the user s responsibility to validate the performance of such product combination for any particular application. Userfriendly software interface the rotorgene offers the most userfriendly realtime analysis software system currently available. This screen appears when a new version of the rotorgene software has been installed and the rotorgene icon is double clicked for the first time after the installation. Kasp chemistry for allelic discrimination performs well on a qiagen rotorgene q instrument and this stepby step protocol will enable users to successfully setup, run and read plates on the qiagen rotorgene q. Page 124 userassigned label associated with the well. This is rotorgene q qiagen by labios on vimeo, the home for high quality videos and the people who love them. Any suggestions for the rotor gene troubleshooting. The optimized kit listed for gene expression at qiagen website is as following for both onestep and twostep pcr. The rotorgene q qiagen real time pcr 5plex hrm system is configured with a highresolution melting analysis, for more information and a wider range of applications. The qiagen rotorgene q is configured with a highresolution melting analysis hrm system, for more information and a wider range of applications.
Replicates run on the rotor gene hand pipetting full 36well rotor full 72well rotor. Rotor gene q mdx user manual, revision r3, for rotor gene q mdx instruments using rotor gene q software version 2. For neutralization of dna of dead organisms 44 pages. Qiagens realtime pcr cycler, the rotorgene q, combines multiple optimized design features to. Developed over the past three years based on customer feedback worldwide, the software has been refined to provide an intuitive, wizard driven interface, enabling highest possible levels of flexibility and. The easy nras kit is designed to be used with the instruments rotorgene q qiagen, rotorgene 6000 corbett research, cfx96 biorad, abi 73007500 applied biosystems and stratagene mx3000pmx3005p agilent technologies. In the rotorgene q software, tm is typically determined by assigning the minimum of the corresponding inverted first derivative curve for details, consult the rotorgene q user manual.
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